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rabbit anti rgc 32 polyclonal igg  (Novus Biologicals)


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    Novus Biologicals rabbit anti rgc 32 polyclonal igg
    Rabbit Anti Rgc 32 Polyclonal Igg, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+rgc32/RGC32+Antibody/pm38878807-79-17-21
    Average 91 stars, based on 2 article reviews
    rabbit anti rgc 32 polyclonal igg - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Incubation:

    Article Title: Response gene to complement-32 promotes cell survival via the NF-κB pathway in non-small-cell lung cancer
    Article Snippet: Subsequently, 20 μg protein from tissues and whole cell lysates was subjected to 12% SDS-PAGE and transferred to PVDF membranes (Bio-Rad Laboratories, Inc.). .. The blots were blocked with 5% fat-free milk at room temperature for 1 h by shaking and then incubated with rabbit anti-RGC32 (cat. no. 4445000; Novus Biologicals, LLC; 1:500) and rabbit p65 (sc-109; Santa Cruz Biotechnology, Inc.; 1:1,000) at 4°C overnight, followed by incubation with horseradish peroxidase-conjugated secondary antibodies (cat. no. 111-035-003; Jackson ImmunoResearch Laboratories, Inc.; 1:200) at room temperature for 1 h by shaking. ..

    Article Title: Response gene to complement-32 promotes cell survival via the NF-κB pathway in non-small-cell lung cancer.
    Article Snippet: Subsequently, 20 μg protein from tissues and whole cell lysates was subjected to 12% SDS‐PAGE and transferred to PVDF membranes (Bio‐Rad Laboratories, Inc.). .. The blots were blocked with 5% fat-free milk at room temperature for 1 h by shaking and then incubated with rabbit anti-RGC32 (cat. no. 4445000; Novus Biologicals, LLC; 1:500) and rabbit p65 (sc-109; Santa Cruz Biotechnology, Inc.; 1:1,000) at 4 ̊C overnight, followed by incubation with horseradish peroxidase-conjugated secondary antibodies (cat. no. 111-035-003; Jackson ImmunoResearch Laboratories, Inc.; 1:200) at room temperature for 1 h by shaking. ..



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    Image Search Results


    Effect of DMU-214 on migration- and proliferation-related genes and proteins in SKOV-3 cells. ( A ) Expression changes of SMAD7, THBS1, IGFBP3, KLF4, IL6, IL1A, SOX-4, IL15, SRF, RGCC and GPR56 genes in cells treated for 24 h with a vehicle or DMU-214 (0.125 μM). RT-qPCR validation of microarray results, presented in a form of a bar graph. Fold Change was presented in its logarithmic form to provide clear comparability of the results. Results of three replicates are presented as mean ± SD. *** p < 0.001, ** p < 0.01 and * p < 0.05. ( B ) Changes of GPR56, RGCC, SRF, THBS1 and SMAD7 proteins level after treated cells for 24 h with a vehicle or DMU-214 (0.125 μM). Western blot was performed to validate microarray and RT-qPCR analysis.

    Journal: International Journal of Molecular Sciences

    Article Title: The Effect of 3′-Hydroxy-3,4,5,4′-Tetramethoxy -stilbene, the Metabolite of the Resveratrol Analogue DMU-212, on the Motility and Proliferation of Ovarian Cancer Cells

    doi: 10.3390/ijms21031100

    Figure Lengend Snippet: Effect of DMU-214 on migration- and proliferation-related genes and proteins in SKOV-3 cells. ( A ) Expression changes of SMAD7, THBS1, IGFBP3, KLF4, IL6, IL1A, SOX-4, IL15, SRF, RGCC and GPR56 genes in cells treated for 24 h with a vehicle or DMU-214 (0.125 μM). RT-qPCR validation of microarray results, presented in a form of a bar graph. Fold Change was presented in its logarithmic form to provide clear comparability of the results. Results of three replicates are presented as mean ± SD. *** p < 0.001, ** p < 0.01 and * p < 0.05. ( B ) Changes of GPR56, RGCC, SRF, THBS1 and SMAD7 proteins level after treated cells for 24 h with a vehicle or DMU-214 (0.125 μM). Western blot was performed to validate microarray and RT-qPCR analysis.

    Article Snippet: Immunodetection was performed with rabbit monoclonal anti-SRF Ab (#5147, Cell Signaling, Danvars, MA, USA), rabbit polyclonal anti-SMAD7 Ab (ab90086, Abcam, Cambridge, UK), rabbit polyclonal anti-THBS1 (ab85762, Abcam), rabbit polyclonal anti-GPR56 Ab (ab172361, Abcam) and rabbit polyclonal anti-RGC32 Ab (sc-84222, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) followed by incubation with goat anti-rabbit HRP-conjugated Ab (#7074S, Cell Signaling).

    Techniques: Migration, Expressing, Quantitative RT-PCR, Biomarker Discovery, Microarray, Western Blot